Achievements for each user project

Baruch Rinkevich

We identified the macrophage like cells, test cells, intestine cells as well as a new population of freely migrating cells which expressed high levels of both DDX4 and DDX3. This last cell population resembles a cell population which was previously described in the literature to be situated in the tunic. Characterization of cells populations expressing high levels DDX4 and DDX3 is of enormous importance for elucidating the functions of these genes, highly targeted by the pharmaceutical industry for therapy.
We also obtained specific staining for actin and tubulin with a specific enhancement in the epithelial cells as well as in the tunic at the fusion area. These results may contribute to our understanding of the processes related to angiogenesis in chordates and vertebrates.

 Even Hjalmar Jørgensen

The project has now finalized the short-term goal; to produce c-DNA from various tissues sampled during one year in sea bass. The project now progress to the medium goal, which is to quantify mRNA expression of the three receptors in all tissues and seasons.

Jamie Stevens

During our stay at the Observatoire Océanologique de Banyuls sur mer (OOBs), we spent two days diving as planned and were able to collect 2 populations and several specimens of conspecific Eunicella and Alcyonium taxa as follows:

1) 29.4.10 Marine Réserve Naturelle de Cerbère-Banyuls
1 population
2) 29.4.10 Cap Béar - Eunicella singularis -1 population
3) 30.5.10 Marine Réserve Naturelle de Cerbère-Banyuls
Alcyonium acaule, A. coralloides, Leptogorgia sarmentosa several individuals of each
4) 30.5.10 Observatoire Océanologique de Banyuls
 Alcyonium acaule 2 specimens

We also collected a specimen of Eunicella verrucosa and Alcyonium palmatum from the OOBs aquarium. We presented our research to OOBs staff and students on April the 27th: Marine connectivity in temperate systems: a molecular approach. From this, we initiated a potential collaboration with an oceanographic modeller and we hope to obtain samples of E. verrucosa from the relevant modeled area with the help of OOBs. These samples will provide important outgroups for both our population genetic and phylogeographic research. The genetic identity of the E. verrucosa colony will be particularly interesting as this species is rare in Banyuls; furthermore, there is thought to be little larval retention in this area. If we can assign this individual to another sample, we could gain insight into rare colonization events into a source area. We will remain in touch with the relevant people and hope to consolidate collaboration once our markers are finalized and when we have some preliminary data for the collected samples. We appreciate excellent organization and hospitality of Dr. Pasal Roman and Dr. Anne-Marie Geneviere who made sure we had a productive and enjoyable stay at OOBs.

Isabelle CARRE

I have learned the transformation technique, which will be required to generate transgenic cell lines carrying epitope-tagged versions of transcription factors. I have also adapted the chromatin immunoprecipitation technique to Ostreococcus . We are now ready to begin the systematic identification of transcription factor binding sites, by chromatin immunoprecipitation using antibodies to the epitope tag.

Amatzia Genin

As planned, we carried out two cruises aboard the OOB´s research vessel during which we sampled zooplankton at 5, 10 and 40 m depth using closing plankton net. Each cruise day was followed by intensive laboratory work, lasting a few days, during which we microscopically sorted the samples collected on the cruises. Based on several tutorial sessions with a local expert on copepods (Dr. Claude Razouls), during which we learnt how to identify the local species and assessed their abundance and distribution, we selected two target species (Calanus and Paracalanus) which were found at the above depths. All the individuals belonging to those species were separated from the samples and photographed using a high-quality microscope with a digital camera. A total of 853 photographs were obtained. The quantity of lipids in each photographed copepod (contained in microscopically visible sacs or drops) will be measured at our home laboratory using standard image processing tools. An additional long plankton haul was carried out at a deep water station (500 m) during the second cruise to sample large, lipid-rich copepods that reside at great depths (down to 200 m). Those copepods were frozen in liquid nitrogen and transported to our home laboratory for further analyses. With the help of the highly-qualified and motivated OOB ship crew, we managed to overcome some loss of equipment (a part of a zooplankton net we brought along). Altogether the planned work was completed in full.