Marine protists & cell lines of marine animals - development, transfection, cryopreservation etc

Primary cultures of functional Atlantic cod melanophores

Detection of glycosaminoglycans and fibrous collagen in marine fish cell lines

Establishing non-axenic monoclonal cultures of Skeletonema marinoi

Detection of mineral nodules in marine fish mineralogenic cell lines

Development of primary cell cultures from calcified tissues of marine fish

 

Nucleofection of marine fish cell lines

Development of primary cell cultures from Ciona intestinalis explant

Preparation of fish serum for the culture of marine fish cell lines

Cryopreservation and revival of marine fish cell lines

Detection of alkaline phosphatase (ALP) activity in marine fish cell lines

 

Development of primary cell cultures from Ciona intestinalis larval stage

Transfection of marine fish cell lines using polyethylenimine (PEI)

Semi-quantitative analysis of cell proliferation in Asterias rubens cell monolayers

Phagocytic behavior of Asterias rubens blood cells or coelomic epithelia cells in vitro

Epithelial cell primary cultures isolated from buds of Botryllus schlosser

 

Preparation of Ascidian hemolymph for the culture of Ciona cells

Preparation of sea urchin gametes and development of sea urchin embryos

Primary cell culture from pituitary of Dicentrarchus labrax

Primary cell cultures from total Paracentrotus lividus coelomocytes

Cryopreservation of marine microalgae employing a controlled rate cooler

 

Cryopreservation of Ectocarpus

Cryopreservation of marine microalgae employing a passive freezer

Automated extraction of PCR-grade gDNA from Ciona intestinalis tissue

Automated whole-mount in situ hybrisization on developmental stages of Ciona intestinalis for the identification of reversive mutations with subtle phenotype

Cryopreservation protocol for Ciona intestinalis sperm

 

Screening protocol for the identification of spontaneous mutations in Ciona intestinalis

Maintenance of marine amoebae

Maintenance of marine ciliates

Isolation of algal endosymbionts from protists

Maintenance of marine heterotrophic protists

 

Use of FlowCam to enumerate and differentiate between marine protists

Elimination of bacteria from microalgal culture using antibiotics

Tips for physical isolation of bacteria-free, clonal microalgae from marine environmental samples

Medium scale culture of micro-algae in polycarbonate carboys

Medium scale culture of micro-algae in plastic bags

 

AFLP analysis as a tool to investigate genetic diversity in microalgae

Molecular barcoding of protists

Culturing of Pseudo-nitzschia species on agar medium

Diatom cleaning with nitric/sulfuric acids

Dinoflagellate isolation from Cnidarian

Microalgae preparation for scanning electron microscopy; dehydration